1,837 research outputs found
Tailoring the magnetic properties of Fe asymmetric nanodots
Asymmetric dots as a function of their geometry have been investigated using
three-dimensional (3D) object oriented micromagnetic framework (OOMMF) code.
The effect of shape asymmetry of the disk on coercivity and remanence is
studied. Angular dependence of the remanence and coercivity is also addressed.
Asymmetric dots are found to reverse their magnetization by nucleation and
propagation of a vortex, when the field is applied parallel to the direction of
asymmetry. However, complex reversal modes appear when the angle at which the
external field is applied is varied, leading to a non monotonic behavior of the
coercivity and remanence.Comment: 5 pages, 7 figure
Preparation of multiplexed small RNA libraries from plants
[EN] High-throughput sequencing is a powerful tool for exploring small RNA populations in
plants. The ever-increasing output from an Illumina Sequencing System allows for multiplexing
multiple samples while still obtaining sufficient data for small RNA discovery and characterization.
Here we describe a protocol for generating multiplexed small RNA libraries for sequencing up to
12 samples in one lane of an Illumina HiSeq System single-end, 50 base pair run. RNA ligases
are used to add the 3¿ and 5¿ adaptors to purified small RNAs; ligation products that lack a small
RNA molecule (adaptor-adaptor products) are intentionally depleted. After cDNA synthesis, a
linear PCR step amplifies the DNA fragments. The 3¿ PCR primers used here include unique 6-
nucleotide sequences to allow for multiplexing up to 12 samples.The original version of this protocol was described in Carbonell et al. (2012). The updated version of the protocol was described in Carbonell et al. (2014). This work was supported by grants from the National Science Foundation (MCB-0956526, MCB-1231726) and National Institutes of Health (AI043288)Gilbert, KB.; Fahlgren, N.; Kasschau, KD.; Chapman, EJ.; Carrington, JC.; Carbonell, A. (2014). Preparation of multiplexed small RNA libraries from plants. Bio-protocol. 4(21):1-17. https://doi.org/10.21769/BioProtoc.1275S11742
The kinematic footprints of five stellar streams in Andromeda's halo
(abridged) We present a spectroscopic analysis of five stellar streams (`A',
`B', `Cr', `Cp' and `D') as well as the extended star cluster, EC4, which lies
within streamC, all discovered in the halo of M31 from our CFHT/MegaCam survey.
These spectroscopic results were initially serendipitous, making use of our
existing observations from the DEep Imaging Multi-Object Spectrograph mounted
on the Keck II telescope, and thereby emphasizing the ubiquity of tidal streams
that account for ~70% of the M31 halo stars in the targeted fields. Subsequent
spectroscopy was then procured in streamCr/p and streamD to trace the velocity
gradient along the streams. For the cluster EC4, candidate member stars with
average [Fe/H]~-1.4 (Fe/H_spec=-1.6), are found at v_{hel}=-285 km/s suggesting
it could be related to streamCp. No similarly obvious cold kinematic candidate
is found for streamD, although candidates are proposed in both of two
spectroscopic pointings along the stream (both at -400 km/s). Spectroscopy near
the edge of streamB suggests a likely kinematic detection, while a candidate
kinematic detection of streamA is found (plausibly associated to M33 rather
than M31). The low dispersion of the streams in kinematics, physical thickness,
and metallicity makes it hard to reconcile with a scenario whereby these stream
structures as an ensemble are related to the giant southern stream. We conclude
that the M31 stellar halo is largely made up of multiple kinematically cold
streams.Comment: 19 pages, 12 figures, accepted in MNRAS. High resolution version,
with fig10 here: http://www.ast.cam.ac.uk/~schapman/streams.pd
The SPLASH Survey: A Spectroscopic Analysis of the Metal-Poor, Low-Luminosity M31 dSph Satellite Andromeda X
Andromeda X (And X) is a newly discovered low-luminosity M31 dwarf spheroidal
galaxy (dSph) found by Zucker et al. (2007) in the Sloan Digital Sky Survey
(SDSS - York et al. 2000). In this paper, we present the first spectroscopic
study of individual red giant branch stars in And X, as a part of the SPLASH
Survey (Spectroscopic and Photometric Landscape of Andromeda's Stellar Halo).
Using the Keck II telescope and multiobject DEIMOS spectrograph, we target two
spectroscopic masks over the face of the galaxy and measure radial velocities
for ~100 stars with a median accuracy of sigma_v ~ 3 km/s. The velocity
histogram for this field confirms three populations of stars along the sight
line: foreground Milky Way dwarfs at small negative velocities, M31 halo red
giants over a broad range of velocities, and a very cold velocity ``spike''
consisting of 22 stars belonging to And X with v_rad = -163.8 +/- 1.2 km/s. By
carefully considering both the random and systematic velocity errors of these
stars (e.g., through duplicate star measurements), we derive an intrinsic
velocity dispersion of just sigma_v = 3.9 +/- 1.2 km/s for And X, which for its
size, implies a minimum mass-to-light ratio of M/L =37^{+26}_{-19} assuming the
mass traces the light. Based on the clean sample of member stars, we measure
the median metallicity of And X to be [Fe/H] = -1.93 +/- 0.11, with a slight
radial metallicity gradient. The dispersion in metallicity is large,
sigma([Fe/H]) = 0.48, possibly hinting that the galaxy retained much of its
chemical enrichment products. We discuss the potential for better understanding
the formation and evolution mechanisms for M31's system of dSphs through
(current) kinematic and chemical abundance studies, especially in relation to
the Milky Way sample. (abridged version)Comment: Accepted for Publication in Astrophys. J. 14 pages including 7
figures and 2 tables (journal format
A Keck/DEIMOS spectroscopic survey of the faint M31 satellites And XV and And XVI
We present the results of a spectroscopic survey of the recently discovered
M31 satellites And XV and And XVI, lying at projected distances from the centre
of M31 of 93 and 130 kpc respectively. These satellites lie to the South of
M31, in regions of the stellar halo which wide field imaging has revealed as
relative voids (compared to the degree-scale coherent stream-like structures).
Using the DEep Imaging Multi-Object Spectrograph mounted on the Keck II
telescope, we have defined probable members of these satellites, for which we
derive radial velocities as precise as ~6 km/s down to i~21.5. While the
distance to And XVI remains the same as previously reported (525pm50 kpc), we
have demonstrated that the brightest three stars previously used to define the
tip of the red giant branch (TRGB) in And XV are in fact Galactic, and And XV
is actually likely to be much more distant at 770pm70 kpc (compared to the
previous 630 kpc), increasing the luminosity from MV -9.4 to MV~-9.8. The And
XV velocity dispersion is resolved with vr =-339+7-6 km/s and sigma-v = 11+7-5
km/s. The And XVI dispersion is not quite resolved at 1sigma with vr =-385+5-6
km/s and sigma-v = 0+10-indef km/s. Using the photometry of the confirmed
member stars, we find metallicities of And XV (median [Fe/H]=-1.58, interquar-
tile range +-0.08), and And XVI (median [Fe/H]=-2.23, interquartile range
+-0.12). Stacking the spectra of the member stars, we find spectroscopic
[Fe/H]=-1.8 (-2.1) for And XV (And XVI), with a uncertainty of ~0.2 dex in both
cases. Our measure- ments of And XV reasonably resolve its mass (~10^8 Msun)
and suggest a polar orbit, while the velocity of And XVI suggests it is
approaching the M31 escape velocity given its large M31-centric distance.Comment: 8 pages, 6 figures, accepted in MNRAS (small revisions from previous
version
Development and characterization of the readout system for POLARBEAR-2
POLARBEAR-2 is a next-generation receiver for precision measurements of the
polarization of the cosmic microwave background (Cosmic Microwave Background
(CMB)). Scheduled to deploy in early 2015, it will observe alongside the
existing POLARBEAR-1 receiver, on a new telescope in the Simons Array on Cerro
Toco in the Atacama desert of Chile. For increased sensitivity, it will feature
a larger area focal plane, with a total of 7,588 polarization sensitive
antenna-coupled Transition Edge Sensor (TES) bolometers, with a design
sensitivity of 4.1 uKrt(s). The focal plane will be cooled to 250 milliKelvin,
and the bolometers will be read-out with 40x frequency domain multiplexing,
with 36 optical bolometers on a single SQUID amplifier, along with 2 dark
bolometers and 2 calibration resistors. To increase the multiplexing factor
from 8x for POLARBEAR-1 to 40x for POLARBEAR-2 requires additional bandwidth
for SQUID readout and well-defined frequency channel spacing. Extending to
these higher frequencies requires new components and design for the LC filters
which define channel spacing. The LC filters are cold resonant circuits with an
inductor and capacitor in series with each bolometer, and stray inductance in
the wiring and equivalent series resistance from the capacitors can affect
bolometer operation. We present results from characterizing these new readout
components. Integration of the readout system is being done first on a small
scale, to ensure that the readout system does not affect bolometer sensitivity
or stability, and to validate the overall system before expansion into the full
receiver. We present the status of readout integration, and the initial results
and status of components for the full array.Comment: Presented at SPIE Astronomical Telescopes and Instrumentation 2014:
Millimeter, Submillimeter, and Far-Infrared Detectors and Instrumentation for
Astronomy VII. Published in Proceedings of SPIE Volume 915
Evidence for seasonal cycles in deep-sea fish abundances: A great migration in the deep SE Atlantic?
Animal migrations are of global ecological significance, providing mechanisms for the transport of nutrients and energy between distant locations. In much of the deep sea (>200 m water depth), the export of nutrients from the surface ocean provides a crucial but seasonally variable energy source to seafloor ecosystems. Seasonal faunal migrations have been hypothesized to occur on the deep seafloor as a result, but have not been documented.
Here, we analyse a 7.5‐year record of photographic data from the Deep‐ocean Environmental Long‐term Observatory Systems seafloor observatories to determine whether there was evidence of seasonal (intra‐annual) migratory behaviours in a deep‐sea fish assemblage on the West African margin and, if so, identify potential cues for the behaviour.
Our findings demonstrate a correlation between intra‐annual changes in demersal fish abundance at 1,400 m depth and satellite‐derived estimates of primary production off the coast of Angola. Highest fish abundances were observed in late November with a smaller peak in June, occurring approximately 4 months after corresponding peaks in primary production.
Observed changes in fish abundance occurred too rapidly to be explained by recruitment or mortality, and must therefore have a behavioural driver. Given the recurrent patterns observed, and the established importance of bottom‐up trophic structuring in deep‐sea ecosystems, we hypothesize that a large fraction of the fish assemblage may conduct seasonal migrations in this region, and propose seasonal variability in surface ocean primary production as a plausible cause. Such trophic control could lead to changes in the abundance of fishes across the seafloor by affecting secondary production of prey species and/or carrion availability for example.
In summary, we present the first evidence for seasonally recurring patterns in deep‐sea demersal fish abundances over a 7‐year period, and demonstrate a previously unobserved level of dynamism in the deep sea, potentially mirroring the great migrations so well characterized in terrestrial systems
The bashful and the boastful : prestigious leaders and social change in Mesolithic Societies
The creation and maintenance of influential leaders and authorities is one of the key themes of archaeological and historical enquiry. However the social dynamics of authorities and leaders in the Mesolithic remains a largely unexplored area of study. The role and influence of authorities can be remarkably different in different situations yet they exist in all societies and in almost all social contexts from playgrounds to parliaments. Here we explore the literature on the dynamics of authority creation, maintenance and contestation in egalitarian societies, and discuss the implications for our interpretation and understanding of the formation of authorities and leaders and changing social relationships within the Mesolithic
Tamoxifen for prevention of breast cancer: extended long-term follow-up of the IBIS-I breast cancer prevention trial
© Cuzick et al. Open Access article distributed under the terms of CC BY.http://dx.doi.org/10.1016/S1470-2045(14)71171-
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